YMC-Accura Triart columns
YMC-Accura Triart columns deliver the proven strength and precision of YMC-Triart resins packed in bioinert columns.
YMC-Accura Triart columns
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YMC-Accura Triart columns deliver the proven strength and precision of YMC-Triart resins packed in bioinert columns. The surface-coated hardware suppresses interaction between analytes and metals in the column’s inner surface and frits. The results are improved peak shapes with high sensitivities, excellent recoveries without the need for column preconditioning, and superior reproducibility with no carry-over effects. YMC-Accura Triart columns are ideal for oligonucleotides, nucleotides, peptides, proteins, metal-coordinating compounds, and highly sensitive LC/MS analyses.
Reliable evaluation of mRNA capping efficiency using LC-MS workflows
This application note describes an optimized ion-pair reversed-phase LC-MS method for evaluating mRNA capping efficiency in therapeutic transcripts. By analyzing short RNA fragments, the approach enables separation and identification of capped, partially capped, and uncapped mRNA species using the YMC Accura Triart Bio C18 column.
The method improves resolution between structurally similar species and supports impurity profiling, making it suitable for quality control of mRNA therapeutics. It also enables differentiation of cap structures such as Cap‑0 and Cap‑1, which are critical for mRNA stability and biological activity.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
Comparison of a chromatographic performance for bioinert HPLC column hardware to standard HPLC hardware
Many large biomolecules, such as nucleotides, proteins/peptides, and metal-coordinating compounds, are prone to surface interactions. These unwanted interactions can have a significantly negative impact on the performance of chromatographic methodology. Over the years, several approaches have been proposed to mitigate the issues caused by these unwanted interactions. A very effective technique is to utilize a surface coating on the column body and frit that makes them bioinert. This scientific poster from YMC America, Inc., details the comparison of chromatographic performance for HPLC hardware with a bioinert surface coating and typical stainless steel HPLC column hardware. Furthermore, the note explains how this coating shows excellent peak shapes for highly adsorptive substances, and how it is especially ideal for use in highly sensitive LC/MS analyses since it prevents carry over and ensures complete recovery without column preconditioning.
