Waters Atmospheric Pressure Gas Chromatography (APGC)

In this application note, Waters present the use of a simple sample extraction and d-SPE clean up where the resulting extract is analyzed by UPLC-MS/MS or APGS-MS/MS in a manner that complies with Canadian regulatory requirements. A single workflow for the multi-residue analysis of pesticides in cannabis is demonstrated.

Helium is the most commonly used carrier gas in gas chromatography, however, finite reserves results in periodic price increases and concern of availability. Nitrogen is a more affordable alternative. This application note highlights the ease of implementing nitrogen as a replacement carrier gas for helium in GC separations, illustrating a cost-effective approach for atmospheric pressure MS sources, while maintaining critical separations and chromatographic performance.

Avocado is a major tropical fruit with various known health benefits when consumed as part of a balanced diet. Its high lipid fraction in particular contains phytosterols, tocopherols, and most notably omega fatty acids. This application note looks to profile the fatty acid content, using gas chromatography following derivatization into fatty acid methyl esters.

This application note describes a method to measure dioxins in a variety of sample matrices using Waters Atmospheric Pressure Gas Chromatography (APGC) with Xevo TQ-S. Dioxins are known to persist in the environment and pose significant toxicological concern, so testing is enforced globally.

A proof of principle method has been established to assess the authenticity of basmati rice using off the shelf supermarket samples with the latest advancements in high resolution GC-MS hardware and informatics. Volatile compounds of interest were extracted from heated dry rice via SPME and headspace. Following a generic GC separation, detection was performed using a Waters® SYNAPT G2-Si MS run in HDMSE mode. Collection of an HDMSE dataset offers a high level of specificity as a result of an ion mobility separation of compounds based on size, shape, and charge, performed after and orthogonal to a GC or UPLC separation.

This application note describes the development and validation of a quantitative method for 141 multi-class POP compounds in a variety of foodstuffs to ensure continued monitoring and consumer safety in Quebec, Canada. The sensitivity, selectivity, and quantification capability of APGC, when coupled with the Waters® Xevo TQ-S is determined, using a generic sample preparation method for the satisfactory extraction of all analytes from a variety of food matrices.

Food safety is a growing worldwide concern, and so is the responsibility of laboratories to meet the increasing demand for monitoring and testing. In this app note discover Water’s solutions to identify diverse chemical compounds, meet compliance requirements, reduce operational costs, and increase productivity.

Various technologies are used to meet the challenge of multi-residue analysis of pesticides in food products destined for human consumption. This application note describes the development and validation of a multi-class method for the routine determination of 142 pesticide residues in various fruit and vegetable matrices.

Multi-residue pesticide analysis is challenging due to the low limits of detection required in a diverse range of food commodities. As there are currently in excess of 1000 pesticides in use, laboratories are under increasing pressure to broaden the range of pesticides determined in ever shorter turnaround times. Therefore, the analytical methods they employ need to be sensitive and robust in a wide variety of sample matrices. This application note demonstrates that the Waters Xevo TQ-S equipped with an APGC source provides a sensitive and repeatable solution for the analysis of pesticides in QuEChERS extracts of strawberry, pear, and spinach.

With the decline in availability and performance limitations of magnetic-sector high-resolution mass spectrometry (HRMS), many laboratories are investigating new methodology and instrumentation to address the demanding needs of ‘“dioxin’” and dioxin-like persistent organic pollutants (POP’s) analysis. In recent years, both the European Union (EU 644-2017 and EU 771-2017) and the United States (USEPA ATP 16130) have recognized the use of tandem quadrupole mass spectrometry (MS) as an acceptable alternative to traditional high-resolution magnetic sector-based methods. Additionally, other countries have evaluated and validated this methodology as a replacement for the traditional HRMS approach, and in doing so, have realized several advantages.

This webinar will discuss:

• Selecting a new instrument to replace legacy HMRS
• How the atmospheric pressure gas chromatography (APGC) MS/MS technology that was selected compared with the previous instrument in performance and usability
• How the new method was accredited

Key learning objectives

• Assessing alternative systems for the analysis of dioxins and furans
• The advantages of tandem MS and atmospheric pressure chemical ionization (APCI) as an alternative test procedure
• Accrediting a new method

Who should attend?

Laboratory managers and senior scientists working with environmental samples for contract testing organizations, regulators, academics

During this webinar, an expert panel will discuss the historical events that have resulted in the consolidation of the use of helium as the GC carrier gas of choice, and how those events may no longer apply to modern instrumentation. The panelists will discuss the benefits of migration to alternative carrier gases, including hydrogen and, more importantly, nitrogen, so that GC and GC-MS laboratories can eliminate their reliance on helium. Due to the cost of helium and increasing supply limitations, this topic is especially relevant, as some have estimated that helium may no longer be available for use in the chromatographic market within a relatively short space of time.

Key learning objectives

• Learn about how the consolidation to helium as the GC carrier gas of choice has come about
• Understand why helium reliance may cause a significant impact on the analytical testing market
• Discover how GC-MS methodologies can be adapted to use nitrogen as a carrier gas, without sacrificing analytical performance
• Understand why hydrogen may not be the best alternative to helium, in terms of GC carrier gas

Who should attend?

• Laboratory analysts
• Laboratory managers
• Laboratory directors

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Watch this video to learn about the benefits of atmospheric pressure gas chromatography as an alternative to traditional analytical techniques. For more information, click on the website link below.

Watch this video to learn about Waters Waters Atmospheric Pressure Gas Chromatography (APGC). Whether you need to reach ultra-trace levels in complex samples or perform a complete UPLC, UPC2 & GC screen to get all the information from your sample, Waters APGC brings advanced MS technologies previously unavailable to GC users.

SelectScience speaks with a panel of gas chromatography experts on the implications of switching to nitrogen as a carrier gas