Transcreener® GDP GTPase Assays
The Transcreener® GDP GTPase Assay Kit directly measures GDP produced during GTP hydrolysis in a homogeneous mix‑and‑read format—no coupling enzymes needed. Compatible with GTPases, GAPs, and GEFs, it supports FP, FI, and TR‑FRET readouts using far‑red tracers to reduce interference. HTS‑ready with excellent sensitivity (Z′ ≥ 0.7), it streamlines screening and profiling workflows.
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The Transcreener® GDP Assay is a universal, mix-and-read HTS assay for detecting GDP, the product of GTP hydrolysis. Designed for GTPases, GAPs, and GEFs, it eliminates the need for coupling enzymes and provides sensitive, interference-resistant results for drug discovery and mechanism-of-action studies.
Key Features & Benefits
- Direct GDP Detection – Measures GDP directly with a highly selective antibody, avoiding coupling reactions and reducing false positives.
- Universal Application – Works with small and large GTPases, GTPase-activating proteins (GAPs), and guanine nucleotide exchange factors (GEFs).
- Multiple Detection Modes – Supports Fluorescence Polarization (FP), Fluorescence Intensity (FI), and TR-FRET readouts.
- Far-Red Tracers – Minimize compound interference and deliver cleaner data in screening libraries.
- HTS Ready – Validated in 96-, 384-, and 1536-well plate formats with robust performance (Z’ ≥ 0.7).
- Automation Compatible – Homogeneous, mix-and-read format with stable signal for streamlined high-throughput workflows.
Applications
- High-throughput screening (HTS) of GTPase inhibitors
- Profiling GAP and GEF activity
- Mechanism of action and selectivity studies
- Hit-to-lead optimization and triage with reduced assay artifacts
