Thermo Scientific™ Evolution™ One/One Plus UV-Vis Spectrophotometers

In this application note, Thermo Fisher Scientific explores how the ability to detect subtle changes in color is crucial in pharmaceutical manufacturing for quality analysis. Variations in color may indicate impurities or degradation in the product, especially for substances prone to decomposition. While qualitative comparison against a standard is common, environmental factors and subjective differences pose challenges. Therefore, spectroscopic measurements, particularly using UV-Visible spectrophotometry, offer a quantitative approach. Overall, color analysis, coupled with advanced spectroscopic techniques, offers a robust approach to pharmaceutical quality assessment, applicable to both liquid and solid samples, and compliant with regulatory standards.

In this eBook, Thermo Fisher Scientific highlights the versatility of the Evolution™ UV-Visible Spectrophotometers. The instruments are effective in measuring the reflection, absorption, and transmission properties of solid materials like films, powders, and curved lenses. The interaction of ultraviolet and visible light with solid materials generates valuable information about the properties and potential utility of materials. By analyzing the degree to which light scatters off the irregular surface of a powder, or how rays of light pass through the shaped lens of an eyeglass, the real-world effectiveness of the materials can be quantified. The four applications described demonstrate their ability to perform routine measurements and generate high-resolution data while providing experimental flexibility for research and more complex applications.

In this application note, Thermo Fisher Scientific presents experimental data on DNA melting using the Thermo Scientific™ Evolution™ One Plus UV-Visible spectrophotometer for absorption measurements with the Quantum Northwest t2 sport peltier cuvette holder to change the sample temperature. The melting temperature was calculated within the Thermo Scientific™ Insight™ Pro software. Determining the melting temperature of dsDNA can be a useful tool for analyzing the composition and structure of the sample. Specifically, this analysis can allow for the identification of irregularities in the DNA sequence, including base-pair mismatches, and can lead to a better understanding of the environmental effects.

In this video, Thermo Fisher Scientific explores mRNA vaccine development. Watch for insights including methodologies, research areas, production, and how to harness UV-Vis spectroscopy for quality assurance and control.

Watch this webinar on demand to discover how absorption and diffuse reflection techniques can be used in the characterization of protein aggregates