SsoAdvanced™ Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.
SsoAdvanced universal probes supermix lets you:
- Carry out high-performance singleplex and duplex reactions — the Sso7d fusion polymerase and advanced formulation enable robust performance in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system
- Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
- Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
- Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
Instrument Compatibility
SsoAdvanced Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of SsoAdvanced Universal Probes Supermix
- qPCR/real-time PCR
- Gene expression analysis
- Absolute quantification
- Genotyping (allelic discrimination)
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Characterization of genetically modified organisms (GMO)
- Genetic profiling
Related Products
We have developed SsoAdvanced™ universal SYBR® Green supermix , which is available in several formats (200–5,000 reactions).
Bio-Rad offers a complete workflow solution for real-time PCR, including:
- RNA isolation kits
- Reverse transcription kits
- Real-time PCR supermixes
More Information
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols . We also provide insights into analysis of qPCR data and troubleshooting reactions .
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
Bio-Rad qPCR Supermixes: Innovative Reagents for Superior Performance
Watch this video to discover Bio-Rad's innovative real-time PCR supermixes. Utilizing advanced formulations and enzyme engineering, they provide optimal real-time PCR data on any instrument, under any conditions, at any time. Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
Improve your qPCR Results with High Processivity Sso7d Fusion Polymerase from Bio-Rad
Watch this video to discover how Bio-Rad’s fusion polymerase has an increased affinity for binding and for the priming site on DNA templates. The double stranded DNA binding domain Sso7d increases processivity through greater binding to the template leading to better handling of challenging samples.
PCR Supermixes: Advanced Enzyme and Buffer Components for Optimal Performance
Watch this video to find out what goes into Bio-Rad’s supermixes. With a diversity of advanced components, Bio-Rad real-time PCR supermixes are ideal for providing optimal data across a broad range of sample types and target regions, including complex sample types. Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
