SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml
1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 µl reactions
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Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.
- Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
- Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
- For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays , self-designed assays, and TaqMan Assays
Product Comparison
* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.
Simple Workflow
Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.
Recommended RT-qPCR Reagents and Assays
Reverse Transcription
- iScript™ Reverse Transcription Supermix for RT-qPCR
- iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
qPCR Supermixes
- SsoAdvanced™ Universal SYBR® Green Supermix
- SsoAdvanced™ Universal Probes Supermix
PreAmp and qPCR Assays
- PrimePCR™ PreAmp Assays
- PrimePCR™ Assays
More Information
Find out more about the features of the SsoAdvanced™ PreAmp Supermix .
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR
Single cell gene expression analysis is a powerful technique that provides a unique and insightful perspective on biological pathways and processes. This application note describes a validated protocol that enables fast and accurate analysis of up to 100 genes in isolated single cells, using multiplex reverse transcription quantitative PCR (RT-qPCR).
Bio-Rad qPCR Supermixes: Innovative Reagents for Superior Performance
Watch this video to discover Bio-Rad's innovative real-time PCR supermixes. Utilizing advanced formulations and enzyme engineering, they provide optimal real-time PCR data on any instrument, under any conditions, at any time. Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
Improve your qPCR Results with High Processivity Sso7d Fusion Polymerase from Bio-Rad
Watch this video to discover how Bio-Rad’s fusion polymerase has an increased affinity for binding and for the priming site on DNA templates. The double stranded DNA binding domain Sso7d increases processivity through greater binding to the template leading to better handling of challenging samples.
PCR Supermixes: Advanced Enzyme and Buffer Components for Optimal Performance
Watch this video to find out what goes into Bio-Rad’s supermixes. With a diversity of advanced components, Bio-Rad real-time PCR supermixes are ideal for providing optimal data across a broad range of sample types and target regions, including complex sample types. Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
