RNAClean XP
The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions.
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The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions. To accommodate various existing laboratory processes, the RNA purified using this chemistry can be run using a variety of IVT/NuGEN labeling kits. Even without the use of organic solvents, centrifugation, or vacuum filtration, RNA is purified in around 20 minutes.
Features:
- Purification of small and large nucleic acid products
- Complete removal of salts, unincorporated primers and dNTPs
- Simple automation-friendly protocol
- No centrifugation, filtration or precipitation steps required
- Elution in aqueous solution
- Purifies both cDNA and cRNA Scalable throughput
Xtra Pure RNA: Agencourt RNAClean XP System
The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions. This method utilizes Solid Phase Reversible Immobilization (SPRI) magnetic bead-based technology. It is uniquely formatted for purification of both the cDNA and cRNA steps in Eberwine based procedures. This technique is easily performed manually in far less time than competitive methods. With the use of SPRI technology, the Agencourt RNAClean XP system doesn’t use organic solvents, vacuum filtration, or centrifugation. The kit delivers superior nucleic acid recovery and purity for use in downstream microarray gene expression experiments.
Solid Phase Reverse Immobilization (SPRI) Bead Technology for Micro RNA Clean Up using the Agencourt RNAClean XP Kit
This application note describes the use of Beckman Coulter’s Agencourt RNAClean XP kit for miRNA clean up and concentration of miRNA and total RNA from low yield samples. The RNAClean XP kit is a Solid Phase Reverse Immobilization (SPRI) paramagnetic bead-based method for RNA and cDNA purification. The magnetic bead procedure does not require centrifugation or filtration steps. It is a simple, rapid, automation-friendly protocol that produces high quality samples.
