Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C Kits
To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.
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Structural proteomics: applications to experimental model of disease
Easy to use. High quality and highly reproducible results. We did not need after sales assistance, but the data sheet is rich with information. It is widely used, which is very important to compare results at high level.
Review Date: 15 Jul 2022 | Promega Corp.
To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.
A Rapid, Automated Immunoaffinity LC-MS/MS Workflow
Promega share its streamlined workflow that involves mAb enrichment, using high capacity magnetic streptavidin particles, followed by proteolysis using a commercially available rapid digestion kit. The rapid enrichment/digestion step, combined with direct LC-MS/MS analysis, allows for a protocol that is quick, simple, and can be easily automated.
