PrismA ELISA kit
The PrismA ELISA kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA ™ chromatography resin and Fibro ™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals.
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The PrismA ELISA kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA™ chromatography resin and Fibro™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals. Purification of mAbs is routinely performed using protein A based affinity chromatography, which provides high capacity and consistent purification performance. When using protein A based purification techniques, it is a regulatory requirement to measure leached protein A ligand in the generated downstream material. For the most accurate results, it is important that manufacturers use a resin-matched standard to calibrate their assay.
Key benefits
- Matched PrismA protein A ligand included in the kit removes the need to source the standard separately and create a custom protocol
- Parts per billion sensitivity minimizes the risk of undetected residual PrismA ligand
- High IgG tolerance simplifies sample preparation
- Consistently low intra- and inter-plate variability ensures reproducible data
- All-in-one kit containing all reagents needed to perform the assay
PrismA ELISA kit
In this application note, Cytiva provides a detailed overview of its PrismA ELISA kit. The kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA™ chromatography resin and Fibro™ PrismA chromatography adsorbers. It is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals.
This kit is the only commercial ELISA kit specifically designed for use with MabSelect PrismA™ resins and Fibro PrismA adsorbers. It contains all the necessary reagents required including the PrismA ligand optimized for use in ELISA, and polyclonal antibodies raised specifically against the PrismA ligand.
