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NEBNext® DNA Library Prep Master Mix Set for 454™

The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation o…

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New England Biolabs Inc.

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The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods.

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the 454 platform.

NEBNext® DNA Library Prep Master Mix Set for 454™ Includes:

  • NEBNext End Repair Enzyme Mix
  • NEBNext End Repair Reaction Buffer (10X)
  • Quick T4 DNA Ligase
  • NEBNext Quick Ligation Reaction Buffer (5X)
  • Bst DNA Polymerase, Large Fragment
  • NEBNext Adaptor Fill-in Reaction Buffer (10X)
  • Molecular Biology Grade Water

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