MitoView™ Fix 640
Far-red, mitochondrial stain with excellent tolerance for fixation and permeabilization after staining.
MitoView™ Fix 640 is far-red mitochondrial stain. The cell membrane-permeant dye rapidly accumulates in the mitochondria of live cells for no-wash, live cell staining. The dye has a reactive group that can covalently attach to proteins inside the mitochondria, so that staining is well-retained after fixation, permeabilization, and subsequent immunofluorescence staining.
- Stain mitochondria in live cells for subsequent fixation
- Well-retained after PFA or MeOH fixation, permeabilization, and IF staining
- Bright, photostable, and non-toxic for long-term live cell imaging
- Cells retain specific staining in culture for days
- Far-red fluorescence for the Cy®5 channel (Ex/Em 648/670 nm)
- Better specificity after fixation compared to MitoTracker® Deep-Red
MitoView™ Fix is not recommended for staining cells that are already fixed, because staining is non-specific in fixed cells. MitoView™ Green dye can be used for fixed cell staining, but for best results with fixed cells, we recommend using one of our mitochondrial marker antibody conjugates.
MitoView™ Fix 640 #70082
MitoView™ Fix 640 #70082-50UG