MiSeq™ i100 Series
The new standard in benchtop sequencing speed and simplicity
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MiSeq is a great tool to have for NGS, its accurate but comes with a cost.
NGS sequencing
MiSeq Illumina for NGS sequencing is a great tool but requires extensive and thorough training, understanding and planning to use the machine. The analysis software is tricky to use, as well the time taken to prepare the library is very time consuming and the kits available to prepare the libraries are kind of expensive to purchase from the 3rd party. If you can afford to have this expensive tool, it's good to have one on site. It certainly adds value to have it, if used efficiently and frequently. It's not cost effective if used rarely and for small number of samples.
Review Date: 9 Apr 2026 | Illumina
The MiSeq i100 Series brings Illumina’s XLEAP-SBS™ chemistry innovation even further, harnessing the potential of room-temperature storage and shipping, which provides customers greater flexibility in how they plan and execute their projects while reducing their environmental impact. It offers flexible output capabilities and comes in two configurations: the MiSeq i100 Plus System has the full 100 million single-end reads per run, and the MiSeq i100 System has a maximum of 25 million single-end reads per run.
Key features include:
- Room-temperature shipping and storage for reagents: Eliminating the cold chain and allowing for greater flexibility to sequence on demand without the need to thaw reagents, which is critical for running urgent samples
- Sustainability: An 85% reduction in packaging waste compared to the MiSeq System supports a lower carbon footprint
- Speed: Dramatic reduction in run times: as fast as four hours, with same-day results (4× faster than MiSeq)
- Cost efficiency: Cost-effective consumables enable more affordable sequencing
- Turnkey workflows: 18 proven end-to-end workflows across 10 applications
- Simplicity: Simpler, streamlined operations for various levels of sequencing experience
For Research Use Only
Not for use in diagnostic procedures (except as specifically noted).
Brochures
Illumina Sequencing Systems: Redefine possible
In this brochure, Illumina highlights its portfolio of next-generation sequencing systems, designed to empower researchers and clinicians across genetic disease, reproductive health, oncology, microbiology, agriculture, and more. Discover how Illumina’s comprehensive suite of platforms provides the tools and innovations needed to generate high-quality genomic data, enabling ground-breaking insights and supporting evolving research and clinical applications.
MiSeq i100 Series: Meet the next generation of benchtop sequencing systems
In this brochure, Illumina introduces the MiSeq™ i100 Series, a fast and easy-to-use benchtop sequencing system designed to simplify next-generation sequencing workflows from library preparation to data analysis. Discover its ability to deliver same-day results for applications such as small whole-genome sequencing, targeted gene sequencing, and gene expression analysis, enabling accessible and reliable genomics research across a wide range of laboratories.
Methods for 16S rRNA sequencing using Illumina platforms
This guide provides an overview of 16S rRNA amplicon sequencing for taxonomic profiling of bacterial communities and microbiomes using Illumina next‑generation sequencing (NGS) platforms. It explains the principles of 16S rRNA gene sequencing, compares amplicon sequencing with shotgun metagenomics, and outlines a step‑by‑step workflow covering library preparation, sequencing, and data analysis for microbiome diversity studies.
MiSeq i100 Series: The new standard in sequencing sustainably
In this infographic, Illumina showcases the MiSeq™ i100 Series, a fast and easy-to-use benchtop sequencing system designed with sustainability in mind. With no bulky boxes, no dry ice, and no need for freezers, the MiSeq i100 Series delivers the fastest, simplest benchtop sequencing with a smaller carbon footprint.
Pathogen sequencing made easy with the MiSeq i100
Tuesday, May 12 at 07:00 BST | 08:00 CEST | 11:30 IST | 14:00 SGT | 16:00 AEST
When a potential outbreak emerges, every hour counts. Rapid pathogen detection depends on sequencing technologies that are fast, reliable, and easy to deploy.
In this SelectScience webinar, held in partnership with Illumina, we will explore how the MiSeq™ i100 was built to support rapid, reliable sequencing for pathogen detection and microbial surveillance. Learn how this next-generation benchtop platform helps laboratories overcome common operational challenges, including cold-chain logistics, limited bioinformatics resources, and long turnaround times.
Through a practical look at real-world microbial sequencing workflows, we’ll demonstrate how the MiSeq i100 enables labs to move efficiently from sample to actionable insight, supporting faster responses to emerging infectious threats.
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Webinar details
- Cost: Free to attend
- Location: Online
- Duration: 60 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
