MACSQuant® X Flow Cytometer
Reliable high-throughput flow cytometry
MACSQuant X Flow Cytometer
MACSQuant X - Liquid Handling Integration
MACSQuant X - Optical Bench
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Fast acquisition, versatile and easy data analysis.
Natural products screening
We use the cytometer for general purposes and, specifically, to run high throughput screening assays for antibiotic and antifungic activity of natural products. It can run plates of 12 up to 384 wells, and even you can define 12-48 eppendorf tubes acquisition setup, meaning that it's very versatile. The speed of run depends on the target, the cell type, the number of events to acquire and the viscosity of the natural product to test (the washing steps between wells, increase). In normal conditions 96 well plate, acquiring 10000 events per well takes 35 minutes in our hands, with less than 0.11 of carryover. It allows us to run libraries of hundreds of compounds per day. The system is robust, and the technical service is very good, keeping the instrument in its best shape. As analysis software we use Flowlogic, that provide fast plate analysis and easy lead detection. MACSQuant X worth its price.
Review Date: 6 Nov 2019 | Miltenyi Biotec B.V. & Co. KG
Engineered from the ground up as a compact flow cytometer, the MACSQuant® X was made to provide speed and reliability in high-throughput screening. It allows you to get more data, in less time, with less effort. This flow cytometer is perfect for maximizing efficiency, while enjoying the certainty of getting reliable results from every tube, 96- or 384-well plate. The MACSQuant X has been designed to meet high-throughput cell analysis needs for the most challenging large-scale screening settings.
Technology highlight
- Integration with liquid handling systems and plate hotels
- Capable of processing up to 9,200 samples per day (96-well plate within 15 minutes, 384-well plate in under 60 minutes)
- Orbital and needle mixing ensure consistency and accuracy in assay performance and cell counting
- 21 CFR Part 11 compliant software option
Product Specs:
- Quantity: EA
- Dimensions: (WxDxH* *adjustable touch screen) 814x474x427-586.5mm
- Applications: Flow Cytometry
- Excitation:
- 405nm (violet)
- 488nm (blue)
- 640nm (red)
- Detection: FSC, SSC, 8 fluorescence channels
- Regulatory status: For research use only
- Sample format: 1.5/2.0/5.0/15/50 mL tubes or 24/48/96/384-well plates
- Measurement speed: < 15 minutes per 96-well plate (5 µL measurement volume per well) Referred value indicates the average of multiple experiments and can differ for individual sample materials. The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode
- Maximum event rate: Up to 15.000 events/second
- Sample uptake volume: 1-5000 µL
- Sample carry-over: 0.1 % Referred value indicates the average of multiple experiments and can differ for individual sample materials. For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 5,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 50 μL at medium flowrate. Plate shaking was set to 1200 rpm and a singlet gate was used to determine singlet counts in originating wells as well as in carry-over wells. The carry-over is defined by sum (CO-singletcount)/sum(SRC-singletcount) ×100%. Results for 384-well plates do not differ significantly from those measured in 96-well plates.
- Flow rate: 25-100 µL/min plus automated flow rate to maintain 500, 1000, or 2000 events/second
- Fluorescence performance: 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales
- Compensation: Automated, or manual with 8x8 matrix, during or after acquisition
- Operating system: Embedded operating system
- Software: MACSQuantify, integrated acquisition and analysis software
- Data files: .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)
- Components: Build-in PC, 15.6” LCD touchscreen, Robotic uptake unit for sample acquisition, Orbital shaker
- Accessories:
- MACS® Chill Racks (5, 15, 50, 96)
- Universal Reagent Rack (130-115-722)
- MACSQuant® Buffer Supply Station (130-101-841)
Brochures
Express Modes for MACSQuantify™ Software: Reliable Data at the Push of a Button
In this brochure, Miltenyi Biotec describes the unique add-on features for the MACSQuantify™ Software. Express modes are standardized data analysis tools that are optimized to automate flow cytometric measurements and analyses via predefined experiment settings, acquisition, and automated gating.
MACSQuant Flow Cytometers - Engineering the Future of Automation
The first MACSQuant® Analyzer was engineered to enable demanding and diverse flow cytometry applications of researchers around the world. At Miltenyi Biotec, we have since continued to expand our instrument portfolio to meet the evergrowing complexities in cell analysis. Innovation is at the core of each MACSQuant Instrument, offering automated flow cytometry solutions for a diverse array of appliations.
Wondering which MACSQuant Flow Cytometer is the best fit for your lab? Explore your options in the MACSQuant family of flow cytometers.
MACSQuant X - Instrument Specifications
The MACSQuant® X has been engineered from the ground up to provide maximum speed and reliability in highthroughput cell analysis. The compact and fully automated, instrument design, taken together with the possibility of modifying your sample format with the click of a button, makes the MACSQuant X the simplest and most powerful solution for high-throughput flow cytometry applications. It allows you to get more data, in less time, with less effort. Perfect for laboratories that want to optimize time while enjoying the certainty of getting reliable results every single run, the MACSQuant X has been designed to meet high-throughput cell analysis needs for the most challenging large-scale screening settings, including, but not limited to, cell therapy research labs, cell manufacturing centers, antibody engineering laboratories, and drug discovery facilities.
The MACSQuant X - Reliable High-Throughput Flow Cytometry
The MACSQuant® X has been engineered from the ground up as a compact flow cytometer made to provide maximum speed and reliability in highthroughput screening. It allows you to get more data, in less time, with less effort. Perfect for laboratories that want to optimize time while enjoying the certainty of getting reliable results every single run, the MACSQuant X has been designed to meet high-throughput cell analysis needs for the most challenging large-scale screening settings.
High-throughput ToxTracker ACE genotoxicity assay on the MACSQuant X flow cytometer
In this application note, Miltenyi Biotec presents a rapid and simple assay for the collection of genotoxicity information . The combination of the ToxTracker ACE assay and MACSQuant X flow cytometer is designed to enable the generation of high-quality, reliable results that are required for compound screening and safety studies in the field of drug discovery and development.
MACSQuant X Analyzer Facilitates Ca²+ Flux Assays
Intracellular calcium (Ca²+ ) serves as a crucial second messenger regulating a variety of physiological processes such as cell division, muscle function, neurotransmission, and vascular contraction. Upon specific stimulation, intracellular Ca²+ concentrations can quickly and significantly increase by the release of internal Ca²+ storages or by entry from the extracellular space through ion channels. Measuring this Ca²+
flux using fluorescent Ca²+ -binding indicators is a widely used method to study cellular responses and to screen receptor or ion channel agonist and antagonist compounds. Flow cytometry–based Ca²+ flux assays can rapidly analyze large numbers of cells at the single-cell level and allow high-content analysis through additional staining to e.g. discriminate dead cells and debris that might interfere with the assay.
Reliable, Hands-Free, and High-Throughput Cell Viability Assays on the MACSQuant Flow Cytometer
The determination of cell viability in any cell culture is a crucial analytical measurement for any study. This includeshigh-throughput cytotoxicity screens of large libraries of compounds and drug discovery research, and studies in all laboratories interested in validating their cell cultures upstream or downstream of any given application.
Accelerating drug development by early unwanted immunogenicity testing
In this video, Miltenyi Biotec discusses how immunogenicity prediction can save precious time when applied in the early phases of drug development. This webinar discusses the best practices in assessing unwanted immune responses and highlights how the MACSQuant® X Flow Cytometer is designed to provide speed and reliability in high-throughput screening.
MACSQuant®X: Revolutionizing Drug Discovery with Automated, Plate- Based Flow Cytometry
Plate-based flow cytometry is enabling scientists to conduct high-throughput screens of individual cells with minimal user-input. In this interview with Andree Salz, Global Product Manager of Miltenyi Biotec, we discover how the unique technology of the MACSQuant® X Platform is revolutionizing screening applications, at every stage of the drug discovery cascade.
This interview was filmed as part of our coverage of SLAS2019 - see other video highlights here >>
High-Throughput 10-Parameter Cell Analysis with the MACSQuant X Flow Cytometer
Find out more about the MACSQuant® X Flow Cytometer from Miltenyi Biotec GmbH
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Cytek Biosciences Debuts Advanced Five-Laser Flow Cytometer
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Miltenyi Biotec to Demonstrate the MACSQuant® X Fully-Automated Flow Cytometry System at SLAS 2019
Miltenyi Biotec will showcase a fully-automated flow cytometry screening set-up at SLAS booth #316
Promising Non-Harmful Imaging Process Acquired by Miltenyi Biotec
New standards set for the optical clearing of biological samples for microscopic analyses
Fast, Flexible and Hands-Free: The Automated Flow Cytometer for the Modern Laboratory
Discover the new MACSQuant X flow cytometer that’s built with speed, accuracy and convenience in mind
