KAPA Library Amplification Kits
The gold standard for NGS library amplification.
Coverage depth vs GC content
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KAPA Library Amplification Kits contain KAPA HiFi DNA Polymerase, a novel enzyme engineered for ultra-high fidelity and robustness using Kapa’s directed evolution technology platform.
KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias. This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.
In addition to the standard library amplification formulation, KAPA HiFi is available in a unique real-time formulation, for applications that demand precise control over library amplification. A uracil-tolerant variant, KAPA HiFi Uracil+ is also available for the high-efficiency, high-fidelity, low-bias amplification of libraries constructed from bisulfite-treated DNA.
KAPA Library Amplification Kits for Illumina platforms now also include an optimally formulated Library Amplification Primer Mix.
Features:
Achieve the lowest amplification bias and duplication rates
- Lower amplification bias result in improved representation of all library fragments and sequence regions
- High-efficiency amplification leads to fewer amplification cycles and lower PCR duplicates
- Less additional next-generation or Sanger sequencing needed to complete genomes
Improve coverage of GC- and AT-rich regions
- Lower amplification bias improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions
- Improved overall coverage and coverage uniformity observed on both Illumina and Ion Torrent sequencing platforms
Exercise precise control over library amplification
- KAPA Real-Time Amplification Kits allow for library amplification to be observed in real time
- Terminate amplification at the optimal point for individual samples
- Optimize library amplification parameters for higher throughput workflows
Amplify NGS libraries with industry-leading fidelity
- KAPA HiFi was engineered using directed evolution to have enhanced proofreading (3′-5′ exonuclease) activity
- Industry-leading fidelity confirmed by 454 sequencing
Applications:
- Amplification of Illumina® libraries (DNA and RNA sequencing)
- Real-time amplification of libraries prepared from low input, ChIP DNA and other precious samples
- Pre- and post-capture amplification in targeted capture workflows
Automation of ChIP-Seq Library Preparation for Next Generation Sequencing on the epMotion<sup>®</sup> 5075 TMX
This application note describes the successful automation of Illumina ChIP-Seq library preparation on the Eppendorf epMotion® 5075 TMX, using the KAPA High-Throughput Library Preparation Kit from Kapa Biosystems. ChIP-Seq library preparation can be a challenging procedure to automate because of its low ChIP DNA input. The combined advantages of the Eppendorf epMotion® 5075 TMX and the KAPA High-Throughput Library Preparation Kit offer an industry-leading solution for standard and low-input library preparation, particularly for ChIP-Seq.
