INVIEW MICROBIOME HIGH SPECIFICITY
High throughput microbial classification down to the species level
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INVIEW MICROBIOME HIGH SPECIFICITY is a next-generation sequencing service that offers characterisation of microbial communities down to the species level based on full-length 16S rRNA gene profiling. The product combines library preparation, sequencing with PacBio long-read technology and reliable BioIT analysis for better separation of species in microbiomes that are difficult to resolve.
Highlights of INVIEW MICROBIOME HIGH SPECIFICITY
- Excellent taxonomical resolution due to high throughput full-length 16S amplicon sequencing
- Classification of microbial communities down to the species level
Brochures
GATC Biotech's Universal Primer List
The following brochure presents GATC Biotech’s universal primer list, for use in PCR and other DNA analyses.
Next-Generation Sequencing: Sample Requirements
The following application note details the sample requirements when performing next-generation sequencing using GATC Biotech instruments.
Full-length 16S rRNA Gene Sequencing Using PacBio RS II
The 16S rRNA gene exhibits an alternating structure of conserved and hypervariable regions which is commonly used for the identification of the bacterial composition of a variety of samples. INVIEW MICROBIOME High Specificity is based on the notion that with a length of about 1,500 base pairs, the full 16S rRNA gene is a perfect candidate for Single Molecule, Real-Time (SMRT) Sequencing with the PacBio RS II system. The current experimental design allows for multiplexing of up to 10 samples in a single SMRT Cell. With a data output of at least 25,000 Reads of Insert (ROI), the total price per sample is drastically reduced.
