INCOGNITO™ T7-FlashScribe™ N1meΨ-RNA Transcription Kit
Specially formulated for maximum yields of N1-methyl-pseudouridine-containing, in vitro transcribed RNA (N1meΨ-RNA). Produce up to 160 µg of N1meΨ-RNA from 1 µg of template in 1 hour. Easily scale up reactions to produce milligram amounts of N1meΨ-RNA. Induce lower innate immune responses in mammalian cells by using N1meΨ-mRNAs.
The INCOGNITO™ T7-FlashScribe™ N1meѰ-RNA Transcription Kit is specially formulated to enable users to obtain the maximum possible yields of N1-methyl-pseudouridine-containing RNA from an in vitro transcription reaction. Although yield varies with the DNA template and other factors, a standard 60 minute, 20 µl reaction will yield up to 160 µg of RNA from 1 µg of the control template. These yields are made possible by the high-performance properties of the T7-FlashScribe™ enzyme. The standard reaction can be scaled up to produce milligram amounts of RNA containing the canonical nucleotides ATP, CTP, GTP and the modified nucleotide N1-methyl-pseudouridine-5'-triphosphate. Kit includes a limited non-exclusive, non-transferable label license to use the products purchased from CELLSCRIPT™ solely for purchaser's own internal laboratory research use. Product is for research use only (RUO).