IMMUNOME™ v4 Discovery Array Service
- High-throughput platform for autoantibody biomarker discovery and protein-protein interactions - Exploratory arrays capable of screening over 1600 proteins in quadruplicate - Picogram level sensitivity - Fully quantitative assay - Exceptional consistency and batch-to-batch reproducibility
IMMUNOME™ v4 Discovery Array is a protein array which utilises the patented Sengenics KREX™ functional proteomics technology. All proteins on the IMMUNOME™ v4 Discovery Array are correctly folded and functional as they are expressed as fusion proteins with biotin carboxyl carrier protein (BCCP) which acts as a folding marker and solubility enhancer. The IMMUNOME™ v4 Discovery Array contains 1600+ full-length human proteins from biologically significant protein families including kinases, signalling molecules, cytokines, interleukins, chemokines and cancer antigens. The arrays are coated with a proprietary surface which preserves protein folding and inhibits binding of non-specific proteins.
1. Discovery of autoantibody biomarkers for diagnostics
2. Discovery of autoantibody biomarkers of risk of adverse drug reactions
3. Discovery of autoantibody-based therapeutic and prophylactic biologics
4. Selectivity screens for new chemical entities and new biologics
5. Functional assays for any protein and any interacting biomolecule
Autoantibody Discovery Using a Novel Microarray of Functional Proteins
Recombinant protein expression is a fundamental technique that underpins clinical diagnostics, drug discovery and screening, vaccine development and pure research for elucidating mechanisms of disease development and progression. However, high-throughput production of correctly folded and functional, full-length human proteins has a very high failure rate. Protein folding is a highly complex process requiring a combination of an aqueous environment, chaperones, post-translational modifications and the formation of multimeric structures held together by covalent bonds. Any deviation from the correct sequence of events can result in a misfolded protein. Loss of protein function is directly linked to misfolding. Use of misfolded proteins in downstream assays and interactions studies can result in identification of false positive biomarkers.
Immunome Protein Arrays: A Step-by-Step Guide
This application note provides step-by-step instructions for carrying out immunome protein arrays.
