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Human CD274 (PD-L1) knockout A549 cell line

Abcam plcab267054

Cell Culture - Human Cd274 KO cell line

Abcam plc

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Description

Cell line: A549

Number of cells: 1 x 106 cells/vial, 1 mL

Cell type: epithelial

Tissue type: Lung

Disease: Carcinoma

Organism: Human

Gender: Male

Antibiotic resistance: Puromycin 1 µg/ml

Adherent: Yes

STR analysis: Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Passage number: <20

Biosafety level: 1

Viability: ~90%

Mycoplasma free: Yes

Storage: Shipped on Dry Ice. Store in liquid nitrogen.

Buffer constituents: : 8.7% DMSO, 2% Cellulose, methyl ether

Application eBookLife Sciences

Knock-out cell lines: A comprehensive guide to use and benefits

Gene knock-out (KO) technology and the generation of KO cell lines has been an essential tool in biology for studying genes of unknown function. However, the advent of CRISPR-Cas9 KO technology is expanding the range of cell lines available for research far beyond previous offerings, rendering them cheaper and more reliable through advancements such as direct injection.

This eBook provides an informative guide on how ready-made KO cell lines benefit workflows, provide quality control, and deliver confidence in results. Download now for insights including:

  • The importance of robust KO validation
  • Sourcing the right KO cell lines for your program
  • gRNAs for increased gene-editing success
  • Investigating signaling pathways such as PD-L1 and TRAIL

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