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HPE™ BlueHorizon™

SERVA Electrophoresis GmbHHPE-BHAvailable: Worldwide

Optimized performance in cooled flatbed electrophoresis like 1D SDS PAGE, 2D electrophoresis, IEF.

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Great instrument. It helped our lab a lot.

 

Average Rating 5.0

Application Area:

Isoelectric Focussing (IEF)

Easy to use, produces good results for a method which is normally not too easy to perform.

Review Date: 16 Jan 2019 | SERVA Electrophoresis GmbH

The HPE™ BlueHorizon™ is a flatbed system for horizontal electrophoresis using precast gels, self-cast gels and gel strips.

Main applications are isoelectric focusing (IEF) including the run of IPG strips (like SERVA IPG BlueStrips) in 2D electrophoresis and SDS PAGE, but also the separation of nucleic acids in polyacrylamide gels. The unit consists of a stable metal housing and an integrated drawer. The drawer holds the cooling plate with connectors for the external refrigeration system (e.g. the circulatory refrigerator bath HPE™ Cooling Unit, cat. no. HPE-CU1).

The cooling plate is made from special ceramic material (maximum gel size 260 x 205 mm) for efficient cooling. It provides even heat dissipation, allowing to run gels at a temperature as low as 4 °C. This is particularly important when applying high voltage to thin isoelectric focusing (IEF) gels. The HPE™ BlueHorizon™ is a very flexible system that could easily be extended to a two-, three- or four-deck system, still under the control of one power supply and one chiller. Stabilizing feets and clamps are available.

Features:

  • High capacity cooling plate
  • For all kinds of film-backed gels
  • Economical reagent usage
  • Smart design - made in Germany
Application NoteSeparations

Precast Gels for Urinary Protein Diagnostics: Electrophoresis of Urinary Proteins with SDS Urine Gels

Proteinuria, the presence of abnormal amounts of protein in urine, can be an indicator of kidney and kidney-related diseases. Detection of proteins in a urine sample can be performed with electrophoresis, this application note describes a method using SDS electrophoresis with polyacrylamide gel, this provides high resolution images and has greater detection sensitivity than agarose gel electrophoresis.


HPE Tower - 2D Electrophoresis on HPE 2D Large Gel

This video shows the complete workflow of the 2nd dimension in 2D electrophoresis (SDS PAGE) using the HPE Tower System. Focused proteins on a SERVA IPG BlueStrip (1st dimension) are separated on a precast HPE 2D Large Gel according to their mass. Click here to see a range of Serva products.

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