GoTaq® Probe qPCR Master Mix
The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format. It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add…
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The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format.
It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired.
The GoTaq® Probe qPCR Master Mix is designed to provide resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs.
Features - Benefits
• Superior Performance: Sensitive detection on any real-time instrument
• Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection
• Versatility: Compatible with both fast and standard cycling methods
• Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors
• Confidence: The Promega PCR Performance Guarantee
Applications
• Sensitive sequence detection and quantification
• Gene expression analysis
• Detection of sequence variants
• Fluorescent detection
Optimized Reagents for Probe-Based qPCR using the GoTaq® Probe qPCR and RT-qPCR Systems
The GoTaq® Probe qPCR and RT-qPCR Systems are optimized for qPCR assays using probe-based detection chemistries. This article presents several experiments performed with the GoTaq® Probe qPCR Master Mix that exhibit sensitive quantification and robust performance.
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