Optimized Reagents for Probe-Based qPCR using the GoTaq® Probe qPCR and RT-qPCR Systems

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® RT-qPCR systems.GoScript™ Reverse Transcription System Features & Benefits: Ultra-Active - Save money on every reaction. Sensitive - Detect rare transcripts. Processive - Transcribe long messages. Resilient - Synthesize cDNA in the presence of strong inhibitors.

The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format.   It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired. The GoTaq® Probe qPCR Master Mix is designed to provide resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs. Features - Benefits • Superior Performance: Sensitive detection on any real-time instrument • Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection • Versatility: Compatible with both fast and standard cycling methods • Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors • Confidence: The Promega PCR Performance Guarantee Applications • Sensitive sequence detection and quantification • Gene expression analysis • Detection of sequence variants • Fluorescent detection