Fluorescence Microplate Assays
Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.
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Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.
Many of these assays include substrates, buffers, and calibration standards as well as kinetic or endpoint protocols.
Fluorescence microplate applications
Caspase Assays
- Caspase-3/7 assays
- Caspase inhibitor-based assays
Cell Proliferation
- DNA content
- DNA synthesis
Cell Signaling and Lipids
- Cholesterol
- Phosphate and pyrophosphatase
- Phosphatase
- Phospholipase
Cell Viability
- Mammalian, yeast or bacterial cell viability
- Multiplexed viability/cytotoxicity assay
Enzyme Activity
- Phosphatases
- Phospholipases
- Proteases
- Other enzymes
β-Galactosidase Assays
- β-galactosidase activity
- β-gal substrates
- Glucosidase substrates
Ion Indicators
- Intracellular calcium
- Intracellular magnesium
- pH indicators
Metabolites and Analytes
- Metabolic assays
- Neurobiology assays
- Inflammation assays
Nucleic Acids
- dsDNA assays
- ssDNA assays
- RNA assays
Protein Quantitation
- CBQCA
- Invitrogen™ Quant-iT™ assay
- Invitrogen™ NanoOrange™ assay
Reactive Oxygen Species
- Oxidative stress
- Nitric oxide
- Other oxidative indicators
Learn more about Fluorescence Microplate Assays by Thermo Fisher Scientific here >>
Interrogating 3D versus 2D cellular models in drug discovery
A current goal in cancer research is to expand the arsenal of quantitative assays that can be used to characterize and interrogate cellular functions in 3D models.
In this application note, Thermo Fisher Scientific highlights how its existing microplate assays can be used to quantify functional differences between 2D and 3D cellular models and provide a time- and cost-efficient method for understanding therapeutic effects on 3D cellular models.
Harnessing the power of cell-based assays: New tools and techniques in 2021
From tackling COVID-19 variants to therapeutic discovery, novel organoids and more, advance your assay research with these exclusive interviews and free resources
