Interrogating 3D versus 2D cellular models in drug discovery

19 Jul 2021

A current goal in cancer research is to expand the arsenal of quantitative assays that can be used to characterize and interrogate cellular functions in 3D models.

In this application note, Thermo Fisher Scientific highlights how its existing microplate assays can be used to quantify functional differences between 2D and 3D cellular models and provide a time- and cost-efficient method for understanding therapeutic effects on 3D cellular models.

Fluorescence Microplate Assays

Thermo Fisher Scientific

Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.

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Interrogating 3D versus 2D cellular models in drug discovery