EvaGreen® Plus Dye, 20X in Water
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
EvaGreen® Plus Dye is an improved alternative to Biotium’s original EvaGreen® Dye. EvaGreen® Plus Dye retains many of the essential benefits of the original patented EvaGreen® Dye, but has an improved signal-to-noise ratio that is more advantageous for a variety of DNA detection applications, including qPCR, digital PCR, and LAMP.
- Lower background & higher signal compared to original EvaGreen® Dye
- Better match for common qPCR instrument excitation sources
- Compatible with qPCR & HRM®
Higher signal to noise offers advantages for digital PCR and isothermal applications - Directly visualize amplification products in gel
Improvements over the Original
Similar to our original EvaGreen® Dye, EvaGreen® Plus Dye is a green fluorescent nucleic acid dye that is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® Plus Dye improves upon these properties with lower background fluorescence and increased brightness upon binding dsDNA. The dye has an excitation maximum that is a better fit for common qPCR instruments, resulting in improved sensitivity. As a result, EvaGreen® Plus Dye can give earlier Ct values and better signal discrimination. These properties offer potential advantages for digital PCR and isothermal amplification applications.
EvaGreen® Plus, 20X in Water, 5x1 mL #31077
EvaGreen® Plus, 20X in Water, 1 mL #31077-T