DNA-free MolTaq 16S/18S & Hot MolTaq 16S/18S
DNA-free Taq DNA polymerases for contamination-free analysis
DNA-free MolTaq 16S/18S
DNA-free Hot MolTaq 16S/18S
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Molzym’s ultra-pure Taq DNA polymerases are highly active and enable ultra-sensitive and reliable PCR-results. MolTaq 16S/18S and Hot MolTaq 16S/18S are especially suited for the PCR/Real-Time PCR analysis of femtogram amounts of bacterial and fungal DNA. Hot MolTaq 16S/18S reduces unspecific amplification and allows the convenient PCR setup at ambient temperature and does not require an extra activation step.
Benefits:
- Highly active Taq DNA polymerases
- Free of contaminating microbial DNA
- Reaction setup at room temperature ( Hot MolTaq 16S/18S)
- PCR enhancer for amplification of GC rich and difficult DNA templates
- High batch to batch quality
- Broad range of applications, e.g. PCR, Real-Time PCR, nested PCR, multiplex PCR, qPCR
The product line of DNA-free PCR reagents also includes eubacterial and panfungal PCR assays, DNA-free water and master mixes for the use with custom primers.
Brochures
Selective Enrichment of Bacterial and Fungal DNA
MolYsis™ is a tool for the removal of human DNA and enrichment of bacteria and fungi from liquid samples. Technically, in a short series of steps, MolYsis™ effects the selective lysis of blood cells and the quantitative degradation (>99%) of released human DNA. After enrichment from the lysate by centrifugation, microorganisms are treated with a reagent degrading cell walls of Grampositive and Gram-negative bacteria and fungi. The broad range lysis potential has been shown in clinical evaluations, including over 200 genera of bacteria (86 Grampositives, 120 Gram-negatives) and 65 genera of fungi.
Hot MolTaq 16S/18S: DNA-Free PCR Reagents
A common drawback of PCR assays for bacterial and fungal sequences is the contamination of amplification reagents by microbial DNA. As a consequence, false-positive results may arise often also associated with a loss of analytical sensitivity. Molzym‘s Mastermix 16S/18S products are guaranteed free of contaminating bacterial and fungal DNA. This provides accurate results also at low target loads.
Rare Pathogen Identification with Molzym's 16S rRNA Gene Diagnosis
In this white paper, Molzym discusses its approach to tackling the current challenges with identifying rare pathogens in clinical diagnostics. With the aim to support tailored antibiotic regimens for patients with critical conditions, the method described, which incorporates 16S rDNA PCR and sequencing analysis, should deliver precise strain identification within hours.
