CyStain PI Absolute P
Reagent kit for nuclei extraction and DNA staining of nuclear DNA from plant tissues to determine absolute and relative genome size and ploidy level. The reagent has been created specifically for selected configurations of the Sysmex CyFlow Ploidy Analyser and CyFlow Space flow cytometers. Kit components: 125 mL Nuclei Extraction Buffer 500 mL Staining Buffer 2 x 1.5 mL Propidium Iodide 1 x 5 mg RNase
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Easy to use, quick and repeatable kit.
Plant ploidiy analysis with flow cytometry
We tried several other options for grass ploidy and genome size analysis including buffers and reagents made in our lab; but the results were not always acceptable. We decided to give this kit a try - it is quick, easy to use and our results (histogram CV-s) were pretty good and repeatable. I'll stick to this kit.
Review Date: 13 Nov 2020 | Sysmex Europe SE
Reagent kit for nuclei extraction and DNA staining of nuclear DNA from plant tissues to determine absolute and relative genome size and ploidy level. The reagent has been created specifically for selected configurations of the Sysmex CyFlow Ploidy Analyser and CyFlow Space flow cytometers.
Kit components:
- 125 mL Nuclei Extraction Buffer
- 500 mL Staining Buffer
- 2 x 1.5 mL Propidium Iodide
- 1 x 5 mg RNase
