Custom Next-Gen Sequencing Oligos
Custom adapters for multiplex next-generation sequencing
Place your order directly with the manufacturer.
Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments. Adapters – especially index adapters since they contain the MID (multiplex identifier) or barcode – containing too high a proportion of truncated sequences (unacceptably low purity) or too high a proportion of other adapter sequences (excessive cross contamination) can lead to compromised sequence read integrity (excessive adapter dimerization too) and improper sequence assembly, respectively, during multiplexing experiments. These types of problems are undetectable until the data analysis stage, which make them costly in terms of time and money. Therefore, the production process used for the adapter sequences is critical for a successful sequencing run. Our custom Next-Gen Sequencing Oligos (NGSO) are manufactured under rigorous conditions to ensure suitable purity and low cross contamination.
Brochures
Custom oligonucleotides: Quality control and quality assurance
In this application note, MilliporeSigma explains why quality control and quality assurance are the top priority for its oligonucleotide chemistry and synthesis platforms.
Oligonucleotide melting temperature
In this application note, MilliporeSigma explains the two methods used to determine the melting temperature of an oligonucleotide, the nearest neighbor method and the basic method.
Oligonucleotide purification
In this application note, MilliporeSigma explains how desalting, reverse-phase cartridge, RP-HPLC, IE-HPLC, polyacrylamide gel electrophoresis (PAGE), gel filtration, and next-generation sequencing can purify oligonucleotides.
Oligonucleotide quantification
In this application note, MilliporeSigma illustrates how to quantify oligonucleotides and explains that the concentration of nucleic acids in solution is readily quantified in a spectrophotometer when exposed to ultraviolet (UV) light at 260 nm.
Protocol for conjugating NHS-ester modifications to amino-labeled oligonucleotides
In this application note, MilliporeSigma describes the protocol for conjugating NHS-ester modifications, such as Texas Red®, to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6.
Traditional HPLC and cross contamination of custom NGS adapters
In this application note, MilliporeSigma discusses how traditional HPLC methods could lead to unwanted (yet undetected) levels of cross contamination, leading to false positives in multiplexing experiments.
Changing the landscape: Disruptive technologies for in vitro diagnostics
The right CMO can provide support to bring disruptive technologies’ potential to fruition
Trusted tools for overcoming next-generation sequencing (NGS) sample prep challenges
Eileen Dimalanta discusses the importance of reliable and trustworthy NGS products and addresses how to overcome current NGS workflow challenges
