Competent Cells

Name: Competent Cells
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For standard cloning experiments competent cells are available in two formats: high efficiency at greater than 108cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 107cfu/μg (HB101). JM109 cells have the additional feature of blue/white screening for recombinants. For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed fo…

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For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli.

Application NoteLife Sciences

High-Yield Plasmid DNA Purification Using PureYield™ Plasmid Miniprep System

This article describes a method for purifying large quantities of plasmid DNA using the PureYield™ Plasmid Miniprep System. Transformed cells grown in enriched medium such as Terrific Broth produce significantly more plasmid using the PureYield™ Plasmid Miniprep System than when grown in standard LB medium.

31 Mar 2015

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Competent Cells

Promega Corp.

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