High-Yield Plasmid DNA Purification Using PureYield™ Plasmid Miniprep System

The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA.   The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. PureYield™ Plasmid Miniprep System Features & Benefits:   Improved Productivity - Rapid protocol purifies plasmid DNA in 10 minutes. Robust Performance - High purity and concentration of plasmid DNA gives proven performance in transfection, cell-free expression and other molecular biology applications. Confidence in Results - Lysis/neutralization indicator dye ensures success every time. Flexible - Centrifugation and vacuum protocols are available.

For standard cloning experiments competent cells are available in two formats: high efficiency at greater than 108cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 107cfu/μg (HB101). JM109 cells have the additional feature of blue/white screening for recombinants. For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression. These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli.