Cogent Phenyl Hydride™ HPLC Columns
Cogent Phenyl Hydride HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a butyl-phenyl ligand that is attached with a direct silicon bond. This makes these non-end capped columns extremely stable with unique separation capabilities that offers great selectivity for polar and non-polar analytes that have aromatic groups.
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The Cogent Phenyl Hydride Columns are the most stable and efficient non-end capped Phenyl Columns on the market. These columns can be used in Reversed Phase, Normal Phase or Aqueous Normal Phase (ANP) HPLC or LCMS.
- Fast Gradients with Short Post Times are possible
- Can be used with pH Gradients, for closely related compounds
- Low Salt Concentration for compound elution when used in ANP Mode
- Phenyl Ring for added selectivity of small Peptides, Sulfonyl, Azide & other compounds with aromatic rings.
These columns have the advantage of fast equilibration between gradients due to their surface properties that will not adsorb water. The extremely low water amount on the surface eliminates the need for long equilibration times even when using in the ANP mode for polar compounds. Precision runs are easily achieved and run cycle times are shorter than standard phenyl columns. When using them in an ANP method (Partitioning as a major retention mechanism is eliminated) the separation is achieved more by interaction with the bonded phase as well as with the silica hydride’s ANP retention mechanism for polar compounds.
Without end-capping, there is no bonded phase bleed which is an excellent benefit when using them with a mass spectrometer. The strong Si-C bonds of the ligand ensure the long-term stability of this phase since they cannot be hydrolyzed and bleed off the column. Use this column when you want different selectivity compared with ordinary phenyl columns.
Coricidin HBP Night Tablet
In this application note, MicroSolv Technology Corporation demonstrates the potential of the Cogent Phenyl Hydride 2.o™ Column for analysis of amine-containing analytes in a real formulation.
Spiked hesperidin extract analyzed with LCMS
In this application note, MicroSolv Technology Corporation describes a method to analyze the components of spiked hesperidin.
Propantheline bromide HPLC Methods
In this application note, MicroSolv Technology Corporation illustrates an HPLC assay method for propantheline bromide tablets in which the need for Ion Pairing Agents is eliminated and the assay can be performed in either the reversed phase or aqueous normal phase mode.
Quinine sulfate and impurity analysis with HPLC
In this application note, MicroSolv Technology Corporation illustrates a method, for Quinine separation from impurity without ion-pair reagents.
Ticagrelor analyzed with HPLC
A rapid, sensitive, and reproducible method has been developed for analysis of Ticagrelor. In this application note, MicroSolv Technology Corporation presents data below that illustrates how the compound can be adequately retained and detected using a simple gradient in reversed phase HPLC.
Sulfonamide antibiotics analyzed with HPLC
In this application note, MicroSolv Technology Corporation presents a method that offers great resolution of a mixture of four Sulfa drugs, antibiotics which are commonly used in both veterinary medicine and also employed to fight disease in honey bees.
Analysis of MDMA in plasma samples with LCMS
In this application note, MicroSolv Technology Corporation presents a highly sensitive method for MS analysis of methylenedioxymethamphetamine (MDMA), for use in forensic research and clinical analysis.
Fluoxetine orthogonal assay method
In this application note, MicroSolv Technology Corporation presents a simple fluoxetine orthogonal assay method that eliminates the requirement for ion-pairing agents.
Hydrocodone with Acetaminophen analyzed with HPLC
In this application note, MicroSolv Technology Corporation presents a simple, robust assay method in which only trifluoroacetic acid is needed in the mobile phase for a symmetrical hydrocodone peak.
Glycosidic analyzed with LCMS
In this application note, MicroSolv Technology Corporation demonstrates how the cogent phenyl hydride column was used to obtain chromatographic separation. The same column can be used in the reversed phase mode as well, highlighting its versatility in the analysis of both hydrophilic and hydrophobic compounds.
