Products & ReviewLife Sciences

CELLCOAT® Protein-Coated Cell Culture Vessels

Greiner Bio-One GmbH Available: Worldwide

Biological coatings on high-quality cell culture vessels

Greiner Bio-One GmbH

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Description

The Greiner Bio-One CELLCOAT® product line comprises cell culture vessels which are coated with proteins of the extracellular matrix (Collagen Type I, Fibronectin, Laminin) or synthetic proteins (Poly-D- and Poly-L-Lysine). Beside an improved adhesion and proliferation of primary cells and various cell lines, CELLCOAT® cell culture vessels are highly suitable for serum-free and serum-reduced cell cultivation and experiments which include additional washing steps or stressful procedures, e.g. transfection. Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.

Catalogue Numbers: XXX950, XXX940, XXX930, XXX920, XXX910

Application NoteLife Sciences

Cultivation and differentiation of human adipose derived mesenchymal stem cells

In this application note, Greiner Bio-One GmbH highlights products from the CELLSTAR® and CELLCOAT® lines, when used in conjunction with StemPro® differentiation kits, as highly suitable for the in vitro differentiation of adipose tissue derived stem cells (ADSCs). Commercially available ADSCs are identified as a valuable stem cell resource that can be efficiently expanded on TC treated CELLSTAR® cell culture labware without compromising their tri-lineage differentiation potential.


Application NoteLife Sciences

Enhanced transfection efficiency on protein-coated microplates

In this application note, Greiner Bio-One GmbH details how transfection efficiency in HepG2, CHO, and HEK-293 cells was assessed using CELLCOAT® plates and reporter plasmids. Results showed varying degrees of cytotoxic effects during transfection, with enhanced cell adhesion observed on PDL- and Collagen type I-coated substrates compared to standard cell culture surfaces. Overall, CELLCOAT® plates demonstrated improved suitability for enhancing cell survival and transfection efficiency.

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