CellBrite® Fix Membrane Stains
Unique fluorogenic membrane dyes that covalently stain the plasma membrane in live cells, and can withstand both fixation and detergent permeabilization.
Receive your quote directly from the manufacturer.
CellBrite® Fix dyes are unique fluorogenic membrane dyes that covalently stain the plasma membrane in live cells, and can withstand both fixation and detergent permeabilization.
- The only membrane dyes that withstand fixation and permeabilization
- Bright, uniform cell surface staining in 15 minutes
- Available with green, red and far-red fluorescence
- CellBrite® Fix 488 validated for super-resolution imaging of exosomes by SIM (Ref. 1)
- Each vial makes 20 uL of 1000X dye
Unique Fixable Membrane Stains
CellBrite® Fix dyes are a series of proprietary fluorophores developed by Biotium to rapidly stain the outer plasma membranes of live cells. They are unique among membrane stains in that their surface staining can withstand permeabilization and methanol fixation, allowing plasma membrane staining to be combined with intracellular staining with antibodies. Other membrane dyes like DiO, DiI, Vybrant® membrane dyes, CellMask™, CellVue®, or PKH dyes can be fixed with formaldehyde. But they are not compatible with detergent permeabilization or methanol fixation, because these treatments extract lipophilic dyes from membranes. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, CellBrite® Fix dyes are general membrane stains.
CellBrite® Fix dyes are designed to accumulate at the cell membrane, where they become covalently attached to membrane proteins. As a result, surface staining is well-retained after permeabilization or methanol fixation, with only a slight increase in intracellular fluorescence compared to formaldehyde fixation alone. CellBrite® Fix dyes are only weakly fluorescent in aqueous media but become intensely fluorescent upon membrane staining. This fluorogenic property of the dyes makes the staining very specific with low background. Due to their better water solubility, CellBrite® Fix dyes yield much more uniform staining compared to lipophilic carbocyanine dyes like DiO and DiI. The dyes are non-cytotoxic and do not readily transfer between cells.
CellBrite™ Fix 555 Membrane Labeling Kit #30088
CellBrite™ Fix 555 Membrane Labeling Kit, Trial Size #30088-T
CellBrite™ Fix 640 Membrane Labeling Kit #30089
CellBrite™ Fix 640 Membrane Labeling Kit, Trial Size #30089-T
CellBrite™ Fix 488 Membrane Labeling Kit #30090
CellBrite™ Fix 488 Membrane Labeling Kit, Trial Size #30090-T
