Bio-Plex® 3D Suspension Array System

The following guide includes a variety of tips and tricks for obtaining optimal results from Luminex multiplex immunoassays, focusing on the Bio-Plex Manager™ software and Bio-Plex® hardware.

Obesity and diabetes are on the rise and constitute a series of complex metabolic disorders with complex interactions among multiple proteins and environmental factors. Murine diabetes and obesity models exist to study these disorders through the measurement of circulating metabolic biomarkers, however, the limited amount of sample poses a challenge to effective analysis. This poster describes a novel multiplex immunoassay that can simultaneously measure eight of the most commonly measured metabolic biomarkers, using the Bio-Plex suspension array system.

Protein phosphorylation plays important roles in signal transduction, from activation of cell surface receptors to activation of transcription factors. To study intracellular signal transduction, it is important to detect the phosphorylation state of a target protein, which is directly related to its activity. This application note describes the performance of two types of multiplex assay panels, which have been validated with carious cell lysates by correlation with western blots.

Cetuximab has been used to treat many advanced cancers, but is only effective in a fraction of patients. In this application note by Bio-Rad the Bio-Plex phosphoprotein assays were used to demonstrate that the effectiveness of cetuximab is not determined solely by its ability to inhibit EGFR activation.

Assay offers largest set of apolipoproteins commercially available