Amicon Ultra-2 mL Centrifugal Filters for Protein Purification and Concentration
With their vertical membrane design, Amicon Ultra 2 mL filters deliver the highest recovery with shortest spin times• Concentrates 2 mL down to 15-70 µl• 98% sample recovery• Fast processing time - 10 to 30 minute spin times• Engineered dead stop• Reverse spin capability provides consistent recoveries
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Protein concentration
Good and easy to use system. Can lose protein on the filter, but similar to most losses.
Review Date: 18 Feb 2015 | Merck
With their vertical membrane design, Amicon Ultra 2 mL filters deliver the highest recovery with shortest spin times
• Concentrates 2 mL down to 15-70 µl
• 98% sample recovery
• Fast processing time - 10 to 30 minute spin times
• Engineered dead stop
• Reverse spin capability provides consistent recoveries
Depletion of Albumin in Rodent Sera Using PureProteome™ Albumin Magnetic Beads
The complexity of serum and the presence of highly abundant proteins like albumin and immunoglobulin can mask less abundant species, hindering biomarker detection. Here, PureProteome albumin magnetic beads are used to remove albumin from mouse, guinea pig and rat sera. . ELISA is used to quantitate removal of albumin in mouse and rat. In addition, a protocol for concentration of these samples using Amicon Ultra 2 mL centrifugal filters is presented.
