AdvanceBio SEC
Agilent AdvanceBio SEC columns ensure easy, fast, and accurate quantitation for mAb aggregation and fragment analysis. AdvanceBio SEC offers two different particle sizes, 2.7 and 1.9 µm.
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Agilent AdvanceBio SEC columns ensure easy, fast, and accurate quantitation for mAb aggregation and fragment analysis. AdvanceBio SEC offers two different particle sizes, 2.7 and 1.9 µm. These innovative size exclusion chromatography columns were designed and engineered by Agilent to achieve high resolution and efficiency separation, with a robust and reliable performance. Consistent results are obtained from column to column, batch to batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.
The AdvanceBio SEC 1.9 µm column is designed to improve resolution and provided faster separation of mAb aggregates and fragments by using both HPLC and UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility, stability as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 µm column is intended to meet the needs for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.
Features:
- High resolution, high-efficiency separation, and accurate quantitation for aggregates and fragments analysis
- Prevented secondary interactions with mAb, ADC, and other proteins
- Assured consistent column performance to eliminate rework
- Enhanced column lifetime and productivity to meet robust CQA analysis
- Specially designed standards for AdvanceBio SEC columns, providing optimal calibration and performance verification
High-throughput and sensitive size exclusion chromatography (SEC) of biologics using Agilent AdvanceBio SEC Columns
In this application note, Agilent Technologies demonstrates the use of shorter and narrower Agilent AdvanceBio SEC columns for fast, high-resolution, sensitive, and reproducible separation and quantitation of monoclonal antibodies (mABs) and antibody drug conjugate (ADC).
Aggregate/fragment analysis
In this application compendium, Agilent Technologies presents a ‘how-to’ guide of size exclusion chromatography (SEC) for biomolecule analysis, detailing key methods and technologies. Discover new ways to elevate your monoclonal antibody aggregate analysis and achieve high-resolution and high-throughput SEC analysis.
High-resolution, high-throughput size exclusion chromatography analysis of monoclonal antibodies
Aggregates and fragments are critical quality attributes of biotherapeutic proteins that need to be well characterized. Size exclusion chromatography (SEC) is commonly used to analyze these size variants. This application note demonstrates the use of the Agilent AdvanceBio SEC 200 Å 1.9 μm column for high-resolution and high-throughput SEC analysis of a monoclonal antibody.
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