ACQUITY UPLC Peptide BEH Columns
Reversed-Phase Peptide Columns
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ACQUITY UPLC Peptide BEH C18, 130Å or 300Å Columns offer a wide usable pH range and outstanding temperature stability for applications ranging from proteomics to peptide mapping. ACQUITY UPLC CSH C18, 130Å Peptide Columns with a low-positive charged surface can be used with standard TFA-containing eluents or with a weaker acid modifier such as formic acid, eliminating the need to compromise between a reversed-phase eluent that delivers sharp peaks or one that minimizes reduction of the MS signal.
Selecting a Reversed-Phase Column for the Peptide Mapping Analysis of a Biotherapeutic Protein
A chromatographic peptide mapping method must provide adequate resolution and recovery of a broad set of peptides if it is to be used in the quality analysis of a biotherapeutic protein. In this application note, a range of reversed phase columns by Waters are analysed for their applicability to peptide mapping, demonstrating high peak capacity separations.
