4titude® FrameStar® 96 Well Non-Skirted PCR Plate (Purple, Clear Wells)
Our FrameStar® PCR plates are designed with patented two-component technology that reduces evaporation from PCR plates, improving your results and allowing you to reduce the volume of expensive reagents to save you money.
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This plate combines the advantages of thin-walled polypropylene wells for maximum heat transferal with a rigid polycarbonate frame to increase the thermal stability of the plate during the PCR process.
The polypropylene wells are the perfect material for PCR, as they are both inert and non-wetting, meaning RNA, DNA and enzymes cannot bind, and the polycarbonate frame eliminates warping due to its thermal stability, meaning that seal the remains unbroken, preventing sample evaporation.
Features:
- Guaranteed free from RNase, DNase and Human Genomic DNA
- Purple polycarbonate frame, clear polypropylene wells
- 0.2ml volume wells, frame cut corner at H1
- Raised well rims for better sealing
- SBS footprint to fit most 96 well blocks
- Autoclavable (121°C)
- Available barcoded
- Frame colour options: purple, blue, green, red, black, clear
- Well colour options: clear, white
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses
The ruminal epithelium is characterized by high transport rates of short chain fatty acids and minerals like Na+, Cl- and Mg2+. However, to maintain stable physiological conditions in the rumen and to meet increased needs of nutrients the transport capacity of the epithelium can even be significantly increased. To investigate the underlying mechanisms of adaptation the expression of several carrier proteins were analyzed under different feeding conditions using the technique of relative quantitative PCR, focussing on changes in gene-of-interest expression rather than on absolute expression.
