Products & ReviewLife Sciences

2D HPE™ Large Gel NF 12.5% Kit

SERVA Electrophoresis GmbH43304Available: Worldwide

Precast gels for large format horizontal 2D electrophoresis (255 x 200 mm).

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Description

2D HPE gels are 650 µm thick – optimised for efficient cooling and staining, without showing overloading effects for highly abundant proteins.

To ensure complete transfer of proteins from the first into the second dimension gel, the composition oft he polyacrylamide matrix of the 2D HPE gels is modified and allows the application of the IPG strip in a sample trench. Shelf-life of the HPE gels is 24 months, and remain stable during storage period. All state-of-the art fluorescent detection (including DIGE and SERVAPurple) can be applied using HPE gels polymerised on non-fluorescent fim-backing (NF). The HPE™ BlueTower™/BlueHorizon™ flatbed electrophoresis devices protect gels from light during the run. No large buffer volumes are needed, the buffer concentrates are included and ready to use.

Features:

  • Horizontal 2D electrophoresis for improved resolution.
  • For IPG strips up to 24 cm length.
  • Homogeneous and gradient gels available.
  • Ready-to-use, all buffers included.
Application NoteSeparations

Precast Gels for Urinary Protein Diagnostics: Electrophoresis of Urinary Proteins with SDS Urine Gels

Proteinuria, the presence of abnormal amounts of protein in urine, can be an indicator of kidney and kidney-related diseases. Detection of proteins in a urine sample can be performed with electrophoresis, this application note describes a method using SDS electrophoresis with polyacrylamide gel, this provides high resolution images and has greater detection sensitivity than agarose gel electrophoresis.



Application NoteLife Sciences

SERVA IMAC and Glutathione Agarose Beads

In this application note Serva explains Immobilized Metal Affinity Chromatography (IMAC) and how it can be combined with IDA (Iminodiacetic acid) agarose beads for easy and fast protein purification.

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