2010 Geno/Grinder®

In this application note, the Geno/Grinder 2010, a bead mill originally designed to smash seeds in deep well plates, was used to disrupt yeast in a microwell plate format.

Mechanical cell lysers are used for effective extraction of genomic DNA from samples containing plant or animal tissue. However, all mechanical lysers do not provide the same outcome. In this application note, the 2000 Geno/Grinder® was compared with a competitive cell lyser (Competitor A) for extraction of DNA from fresh basil.

The isolation of nucleic acids from intact seeds requires mechanically disrupting the seed followed by the extraction and subsequent purification of the nucleic acid. In this application note, different methods of mechanically disrupting the seeds are discussed.

The high-throughput analysis of pesticides has been hindered by the slow and laborious sample preparation stage. Using traditional clean-up procedures, over twenty steps are required to get the sample in a convenient form for analysis, which limits sample throughput to typically 8 samples per day. This application note, evaluates a new approach using an innovative laboratory tissue homogenizer, to extract various pesticide residues from different kinds of plant materials and to identify and quantitate the active ingredients by LC/MS/MS. It shows that by automating the sample preparation procedure, the number of clean-up steps can be significantly reduces, resulting in an increased sample throughput over traditional approaches.

In this application note, samples of strawberry, apple, and green pepper were prepared for analysis using both the standard QuEChERS method and a modified method involving mechanical mixing using the Geno/Grinder. The Geno/Grinder is a mechanical disrupter that grinds and mixes materials using a rapid vertical motion. GC-MS results of samples prepared using both methods were compared.

In this application note, the Geno/Grinder was employed to homogenize the fruit/vegetable samples and to mix the produce rapidly and thoroughly with the salts and solvent in an effort to improve the extraction step. The goal of the study was to determine whether the use of the Geno/Grinder during the extraction step would increase pesticide recovery over the traditional, manual QuEChERS method.

The mechanical homogenization for extraction of fatty acids is traditionally performed with an Ultra-Turrax in a buffer solution. However, both the purification and the slow sample preparation process in the analytical laboratory are labour-intensive. This method is no longer suitable for high sample throughput. This application note demonstrates that the Geno/Grinder 2000 is well suited for cell disruption of muscle and internal organs, i.e. liver.

This application note describes the homogenization of sugar beet leaves for the purpose of extracting and isolating DNA for PCR amplification. The Geno/Grinder 2000 was used for the high throughput processing of sugar beet leaf material.

This application note describes a quick DNA extractions method that produces a high enough concentration of DNA that can be used for PCR.

The high-throughput analysis of pesticides has been hindered by slow and laborious sample preparation. This application note evaluates a new approach using an innovative laboratory tissue homogenizer to extract various pesticide residues from different kinds of plant materials and to identify and quantify active ingredients by LC/MS/MS.

This video guide demonstrates the varied applications of the 2010 Geno/Grinder® from Spex SamplePrep, allowing for effective, automated homogenization of large numbers of samples. Discover the settings and applications available in this adaptable machine.