Next Generation Sequencing Products & Reviews

Accurate and sensitive quantification of DNA, RNA, and protein with flexible throughput

Agilent’s market leading SureSelect platform provides a complete portfolio of catalog and custom products, providing the flexibility you need from discovery to follow-up studies. Agilent’s custom solutions allow you to target your regions of interest using our free web portal SureDesign. Custom solutions are available for DNA and RNA target enrichment.

Agilent's SureSelect market leading platform provides a complete portfolio of catalog and custom products, providing the flexibility you need, for your discovery to follow-up studies. Agilent™s SureSelect Target Enrichment System is a highly efficient hybrid selection technique for optimizing next-generation sequencing. It is available for the Illumina GA and HiSeq, Applied Biosystems SOLiD, and 454 Life Sciences FLX and GS J…

The SureSelect XT HS2 DNA Reagent Kit is Agilent’s most cutting-edge library prep and target enrichment solution for Illumina sequencing

The future of NGS library preparation is here. 

The NGS STARlet is a compact, flexible and future-proof workstation for library preparation for all common sequencing platforms. It is excellently suited for any throughput from 8 to 48 samples and reduces time consuming manual pipetting steps and sample-to-sample variability. The open workstation concept allows automation of any library preparation chemistry.

Twist Custom Panels allow customers to create a customized NGS target enrichment panel that targets their specific regions of interest. Available for DNA, RNA, and methylation workflows, our bioinformatics team will help you design a panel tailored to your needs - created with the same double-stranded biotinylated probes that enable the exceptional performance of our fixed panels.

Twist Bioscience, the leader in synthetic DNA with unparalleled precision at scale, is redefining targeted sequencing performance with the Twist Human Core Exome Kit. This kit includes library preparation and enrichment components, allowing the flexibility to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

The Twist Human Comprehensive Exome Panel offers over 99% coverage of protein-coding genes, providing a solution for exome sequencing with high performance and flexibility.

Positive controls provide quality control measures for the verification and validation for point-of-care diagnostic tests including both next-generation sequencing (NGS) and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Twist offers fully synthetic SARS-CoV-2 RNA controls, available for dozens of distinct reference sequences.

The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases

Twist Library Preparation Enzymatic Fragmentation (EF) Kit 2.0 is built to help you achieve a more efficient sequencing pipeline and obtain more accurate Next-Generation Sequencing (NGS) results. This updated kit has new fragmentation and ligation modules, and includes the Equinox Library Prep Amp Mix. This hot-start enzyme formulation has a lower error rate* and high efficiency at low input volumes—making it ideal for samples…

~4x more coverage of all CpG sites

Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Also, the copy control vector can be used for easy DNA purification from fosmid clones. Fosmid libraries are commonly used for closing gaps in a whole genome se…

Illumina's MiSeq is the only fully integrated personal sequencer, delivering a streamlined solution that takes you from rapid sample prep through automated data analysis and storage in the BaseSpace cloud. Sequencing doesn’t get any easier than this. As the only personal sequencer capable of producing paired-end reads, Illumina's MiSeq system puts the largest portfolio of sequencing applications at your fingertips. Optimized…

Fast and affordable next-generation benchtop sequencer

SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing.

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…

he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.NEBNext® reagents are a series of highly pure reagents that facilitate samp…

NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…

The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents…

Next-generation sequencing (NGS) technologies have dramatically reduced sequencing costs and led to novel approaches in many fields of biology.

The MiSeqDx instrument is the first and only FDA-cleared in vitro diagnostic (IVD) next-generation sequencing (NGS) system. Designed specifically for the clinical laboratory environment, the MiSeqDx instrument offers a small, approximately 4 square feet (0.37 square meters) footprint, an easy-to-follow workflow, and data output tailored to the needs of clinical labs. In addition, the integrated software enables sample trackin…

Our latest generation Ovation RNA-Seq System V2 provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications, now with enhanced transcript coverage and uniform distribution of sequencing reads. Amplification is initiated at the 3' end as well as randomly throughout the whole transcriptome in the sample. This feature makes the Ovation RNA-Seq System V2 ideal for amplification prior to…