The LC handbook

2 Jul 2026

This handbook from Agilent Technologies provides a practical introduction to liquid chromatography (LC), with a particular focus on reversed-phase high-performance liquid chromatography (HPLC). Designed as a reference guide for chromatographers of all experience levels, it covers the fundamentals of chromatography, column selection, packing materials, fittings, troubleshooting, and methods for improving productivity and analytical performance.

The guide aims to help users choose the most appropriate LC column for their application while addressing common challenges encountered during method development and routine analysis. The handbook also includes practical tips, application guidance, and troubleshooting advice to support work in analytical laboratories across industries including pharmaceuticals, environmental testing, food and beverage analysis, and chemical research.

Resource details:

Resource type: Application notes
Pages: 200
Read time: 4 hours

Related products

Request Quote for All Products

Agilent 1290 Infinity II LC System
Agilent Technologies
The 1290 Infinity II LC System is an ultra-high performance LC that has market-leading sample capacity and is designed for the lowest dispersion for the most challenging ultra-HPLC experiments. The 1290 Infinity II LC system is designed to be both a HPLC and high-end UHPLC system in a single flow path, giving you robust and excellent performance no matter what application you are running while seamlessly integrating into your existing laboratory environment.
(91)
Agilent 1290 Infinity III High-Speed Pump
Agilent Technologies
The 1290 Infinity III High Speed Pump uses high-pressure mixing for UHPLC gradient formation and solvent blending from up to two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.
(0)
Poroshell 300
Agilent Technologies
Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks.
(1)
AdvanceBio RP-mAb
Agilent Technologies
Agilent AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications
(0)
AdvanceBio Peptide Mapping
Agilent Technologies
Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence.
(0)
AdvanceBio Glycan Mapping
Agilent Technologies
Agilent AdvanceBio Glycan Mapping Columns are designed and manufactured to deliver FAST, high resolution, reproducible glycan identification using HILIC chromatography.
(0)
AdvanceBio Oligonucleotide Columns
Agilent Technologies
To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.
(0)