Agilent 1290 Infinity III High-Speed Pump
The 1290 Infinity III High Speed Pump uses high-pressure mixing for UHPLC gradient formation and solvent blending from up to two solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.
The 1290 Infinity III High Speed Pump has an exceptionally low delay volume, which allows you to run ultrafast gradients for narrow-bore UHPLC applications. This capability makes this pump the ideal front-end for LC/MS applications. The full ISET range allows you to seamlessly transfer existing methods from different instruments, including current Agilent systems as well as instruments from other manufacturers.
Features
- Power range combines ultrahigh pressure up to 1300 bar and high analytical flow rates up to 5 mL/min for maximum UHPLC performance
- Lowest delay volumes down to 10 µL allow you to run ultrafast gradients on narrow-bore columns, making this pump the ideal front-end for LC/MS applications
- Intelligent System Emulation Technology (ISET) enables emulation of existing Agilent 1100, 1200, and 1260 Infinity Series instruments, as well as Waters Alliance, Waters H-Class, and Shimadzu Prominence instruments facilitating seamless method transfer
- Active damping with independently controllable high-resolution pump drives and firmware-embedded tuning algorithms significantly reduce ripples and associated UV noise
- Multipurpose valve enables automated software-embedded functionality such as switching the optional mixer in and out, flushing back the inline filter or automated purging for reduced manual interaction
- Jet Weaver mixer, based on multilayer microfluidic technology, combines highest efficiency mixing with lowest delay volume for highest UV-detector sensitivity
- Integrated high-efficiency degasser with low internal volume is based on PTFE AF technology and provides for fast change-over of solvents for purging and priming the pump
- Built-in active seal-wash increases instrument uptime
Reduce PFAS background with the Agilent PFC-Free HPLC conversion kit
Per- and poly-fluoroalkyl substances (PFAS) can cause increased background, when using LC-MS/MS instruments, which can have a severe impact on meeting the required quantification and detection limits set by regulations. Therefore, it is up to the user to take the necessary steps to minimize its impact on the analytical results.
In this technical overview, Agilent Technologies demonstrate how the PFC-free HPLC conversion kit can minimize PFAS background from LC instruments and support customers running PFAS applications, making it an ideal solution for trace level PFAS analysis.



















