HybridSPE(TM)-Precipitation Technology Wins SelectScience.net 2009 Scientists' Choice Award for Best New Separations Product in 2008
7 Apr 2009Product news
Supelco, a division of Sigma-Aldrich, and a leading supplier of chromatography products for analysis and purification, announces that its HybridSPE-Precipitation technology has won the SelectScience.net 2009 Scientists' Choice Award for Best New Separations Product in 2008. SelectScience.net is one of the leading online resources for applied chemists, clinical chemists and life scientists. The Scientists' Choice Award was based on an independent poll of over 25,000 scientists within the SelectScience.net community. The award was announced at Pittcon 2009 held recently in Chicago, IL.
"The advent of LC-MS technology has resulted in a wealth of benefits in terms of analysis speed and sensitivity, but sample prep still was a bottleneck within the overall bioanalytical process," said An Trinh, Product Manager of Sample Prep at Supelco. "Our goal was to deliver a new and innovative sample prep platform that would be simple enough in use to employ in a high throughput setting while simultaneously improving the overall quality of the researchers' data. We are very happy to learn that our efforts have been recognized by the scientific community."
HybridSPE-Precipitation (HybridSPE-PPT) technology is a simple and generic sample preparation platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis.
In pharmaceutical bioanalysis, researchers develop and run various assays to quantitate drugs, pharmaceutical candidates, and metabolites in biological fluids such as serum and plasma. Excessive background from endogenous matrix components has always been a great concern in quantitative bioanalysis, and has become paramount with decreasing analytical run times. In bioanalytical mass spectrometry, the issue of excessive background contributes to the growing problem of ion-suppression.
One of the major causes of ion-suppression in bioanalysis is the presence of phospholipids during LC-MS or LC-MS-MS analysis in the positive ion electrospray mode (+ESI). Phospholipids are the second largest lipid component in biological matrices after triglycerides, and are typically present in extremely high concentrations in biological plasma samples.
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HybridSPE®-Precipitation, 96-well Plate, pk of 1
Sigma-Aldrich SupelcoPatent pending HybridSPE – Precipitation (HybridSPE-PPT) technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-PPT 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-PPT stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis. An alternative “In-well Precipitation” method is available for the HybridSPE-PPT 96-well version in which biological plasma/serum is first added to the 96-well plate followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied to the 96-well plate. Because the 96-well version contains a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process.