Enhanced AAV capsid protein analysis using CE-SDS with native fluorescence detection on the BioPhase 8800 system

This resource highlights AAV capsid protein analysis using CE‑SDS on the BioPhase 8800 system, enabling high‑resolution separation and quantitation of VP1, VP2, VP3, and VP3 capsid proteins. Comparative results using UV, laser‑induced fluorescence (LIF), and native fluorescence (NF) detection show that NF and LIF provide improved sensitivity, flatter baselines, and more reliable peak integration than UV detection.

The workflow also demonstrates how CE‑based assays for plasmid, RNA, and capsid analysis can be combined on a single platform to support comprehensive AAV critical quality attribute (CQA) assessment throughout gene therapy development and production.