Improved Reproducibility and Reduced Sample Preparation Time for the HPLC Analysis of Aflatoxins in Raw Peanut Paste

Fast and Simple Cleanup for Mycotoxin Analysis.The need for a quick, simplistic sample cleanup approach prior to chromatographic mycotoxin analysis has brought about SPE cartridges that significantly decrease sample prep time, increase reproducibility, and are more user friendly as compared to the industry standard immunoaffinity columns. In addition the Supel Tox SPE approach requires less equipment and fewer consumables.Unlike the multiple step "bind and elute" strategy implemented when using immunoaffinity columns, the Supel Tox AflaZea, DON, and Tricho SPE cartridges employ an "interference removal" strategy which saves time by eliminating wash steps prior to elution of aflatoxin, zearalenone, deoxynivalenol, and tricothecenes (type A and B), respectively. Cartridges removing interferences associated with analysis of the above and fumonisins (B1 and B2) as well as ochratoxin A are also available as a part of our Supel Tox product offering.Features and Benefits:• Better reproducibility than the current industry standard, immunoaffinity columns• Removes interferences associated with mycotoxin analysis• Basic and quick methodology, requires little additional method development• Sample preparation time is ten times faster than when using immunoaffinity columns• Improved shelf life over immunoaffinity columns due to thermally stable format• No refrigeration required for shipping and storage

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.