Application Note: Easily Measure Extracellular Acidification Rate (ECAR)
20 March 2019

This product flyer details how the pH-Xtra Glycolysis Assay allows researchers to conveniently assess glycolytic activity for metabolic characterization and determine how specific interventions like nutrient deprivation, hypoxia, drug treatment or genetic manipulation impact on glycolytic flux. It is shown how, unlike conventional end-point glycolysis assays, the pH-Xtra Glycolysis Assay measures extracellular acidification rate (ECAR) in real time, allowing the observation of temporary changes and fast responses of the glycolytic machinery. The samples used are unsealed; under these conditions, ECAR is found to be a reliable and robust measure of glycolytic activity.