Application Note: Determining Mechanisms of Mitochondrial Toxicity using Agilent Seahorse XF Technology
22 March 2019

In this poster, a standard workflow is presented to enable a comprehensive characterization of the mechanism of action of mitotoxic compounds. Using the XF Real-Time ATP Rate assay in intact cells (HepG2), several compounds with mitotoxic  were identified, but not cytotoxic effects after acute (1 hr) or chronic (18 hr) treatment.