Application Note: Agilent 6475 LC/TQ performance highlights with EPA draft method 1633 for per and polyfluoroalkyl substances (PFAS) in solid samples
11 September 2023

In this application note, Agilent Technologies details the performance of its 6475 Triple Quadrupole LC/MS (LC/TQ) system coupled with the Agilent 1290 Infinity II liquid chromatograph (LC) system for the analysis of per- and polyfluoroalkyl substances (PFAS) in accordance with the third draft of EPA 1633. Method transfer between the Agilent 6475 and 6470 B triple quadrupole LC/MS systems was seamless, and the new intelligent source optimization algorithm was tested, as the EPA 1633 analyte list includes thermally sensitive compounds. Calibration linearity and reproducibility were verified, and the results met all method requirements. The same soil extracts were analyzed on both systems (6470 B and 6475 LC/TQ) to demonstrate comparable performance. Finally, instrument robustness was challenged by >1,000 noninterrupted injections of extracted fish samples while monitoring calibration verification standards after every 50 injections of matrix.