TechTalk: How adaptive amplification transforms NGS library prep for complex samples
Friday, June 26 at 16:00 BST | 17:00 CEST | 11:00 EDT | 08:00 PDT
Working with cfDNA, FFPE tissue, or single-cell samples means working with material that is precious, often irreplaceable, and highly sensitive to amplification errors. Yet most NGS library prep workflows still rely on fixed-cycle PCR, applying the same cycle count to every sample regardless of input quality or quantity. The result? Over-amplified libraries packed with duplicates and artifacts, or under-amplified samples that never make it to sequencing.
In this 20-minute TechTalk, held in partnership with n6, Sr. Field Application Scientist Mary Arrastia walks through why uncontrolled PCR amplification is the biggest threat to data quality in difficult sample workflows, and how AutoNorm™ adaptive amplification technology solves it.
With a live instrument tour, software demonstration, and application data from cfDNA, FFPE, and single-cell workflows, you will see exactly how iconPCR™ gives every sample the precise amplification it needs, automatically.
Certificate of attendance
If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.
TechTalk details
- Cost: Free to attend
- Location: Online
- Duration: 20 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
Speakers
Who should attend?
- Researchers, lab managers, and genomics core scientists who regularly work with challenging sample types in NGS workflows
- Liquid biopsy / cfDNA for MRD monitoring, early cancer detection, and rare variant calling
- FFPE-derived samples in oncology, pathology, and clinical genomics
- Single-cell sequencing on 10x Genomics or similar platforms with variable cell capture
- Any low-input or degraded DNA/RNA application where library prep failure is costly
- NGS core facilities, managing diverse sample types across multiple users and projects
Featured products
Request Quote for All Products
icon96™
n6 Tec., IncStop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.
icon16™
n6 Tec., IncThe icon16™ brings AutoNorm™ adaptive amplification to lower-throughput NGS workflows, with up to 16 samples at a time. Designed for labs that refuse to compromise on data quality, it delivers per-well real-time fluorescence monitoring and automatic library normalization in a compact, benchtop footprint.
What will this webinar cover?
- Understand why fixed-cycle amplification fails for variable, low-input, and degraded samples, and how uncontrolled library prep directly impacts downstream sequencing data quality.
- Learn how AutoNorm™ adaptive amplification monitors each library in real time and stops every well at its optimal yield endpoint, eliminating manual normalization steps from your NGS workflow.
- See application data demonstrating iconPCR™ library prep performance across difficult sample types including FFPE (variable input and degradation), cfDNA (ultra-low input, rare variant detection), and single-cell sequencing (variable cell capture).
- Discover how iconPCR™ integrates into existing NGS library prep workflows without proprietary reagents, kits, or protocol changes.