Testing Pippin Prep Usability for ChIP-Seq Libraries
1 Dec 2011
This video highlights the use of the various tests preformed to ensure that the Pippin Prep machine is fully operational and fully optimized. TF ChIP-Seq and Histone Mark ChIP seq libraries utilize the Pippin Prep. The Pippin Prep automates preparative gel electrophoresis for the construction of next gen sequencing libraries. By setting a new standard for sample quality of size-selected DNA, effort is reduced, bioinformatics are improved, and costly sequencing runs are more fully utilized.
About the company
Sage Science
Sage Science develops game-changing instrument products for sale to the Life Sciences research and Diagnostic laboratory markets. Our mission is to provide new systems to streamline and improve sample preparation workflows.
Sage Science is privately-funded. It is headquartered and manufactures at the Cummings Center in Beverly, MA, USA.
The Company released the Pippin Prep DNA size selection system in 2010. The Pippin has since established itself as the premium platform for high quality library construction for next gen sequencing applications.
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Pippin Prep DNA Size Selection System
Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.
